Yeast extract 0.50 g
Proteose Peptone (Difco no. 3) 0.50 g
Casamino acids 0.50 g
Glucose 0.50 g
Soluble starch 0.50 g
Na-pyruvate 0.30 g
K2HPO4 0.30 g
MgSO4 x 7 H2O 0.05 g
Agar 15.00 g
Distilled water 1000.00 ml

Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121° C.